Why is methylfolate the off-switch for the glycine buffer system? | Masterjohn Q&A Files #239

Why is methylfolate the off-switch for the glycine buffer system? | Masterjohn Q&A Files #239

Author: Chris Masterjohn, PhD July 21, 2021 Duration: 4:15

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Question: Why is methylfolate the off-switch for the glycine buffer system?

The reason is that generally in the fed state, you have lots of incoming methionine. And so you have an excess of SAMe in the fed state. In the fasting state, you have no incoming methyl groups from diet, and therefore the fasted state is the low-methylation state. So fed state, high methylation state, fasted state, low methylation state. You basically shut off MTHFR in the fed state, because in the fed state, which is a high methylation state, the incoming methionine makes you not need any MTHFR activity.

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DISCLAIMER: I have a PhD in Nutritional Sciences and my expertise is in performing and evaluating nutritional research. I am not a medical doctor and nothing herein is medical advice.

Access the show notes, transcript, and comments here.

Chris Masterjohn, PhD, is the Founder and Scientific Director of the mitochondria test Mitome.


Chris Masterjohn, PhD hosts Mastering Nutrition, a podcast that digs into the science of how our bodies work at a fundamental level. The focus here is on mitochondrial health-the energy powerhouses within our cells-and how optimizing them forms the cornerstone of lasting vitality. Chris brings his background as a nutrition scientist and his experience founding BioOptHealth to these conversations, which move beyond generic diet advice. Instead, the podcast explores how individual differences, informed by tools like whole genome sequencing and detailed biochemical data, can reveal personalized paths to better metabolic function. Listeners will hear deep dives into cutting-edge research, practical interpretations of complex studies, and discussions on how to apply these insights in a real-world context. This isn't about quick fixes or trending superfoods; it's about building a coherent, scientifically-grounded understanding of nutrition from the cellular level up. Each episode aims to provide the kind of nuanced, evidence-based perspective that can help you make sense of conflicting health information. Whether the topic is a specific nutrient, a metabolic pathway, or a broader principle of wellness, Chris works to translate dense science into actionable knowledge. Tune in for a thoughtful, detailed exploration of what it truly means to master your own nutritional landscape.
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